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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 10 Documents
 1 
Search results for , issue "Vol 4, No 1 (2014)" : 10 Documents clear
Morphological And Biochemical Responses Of Saccharum Spontaneum L. Accessions To Drought Stress Munawarti, Aminatun; Taryono, Taryono; Semiarti, Endang; Sismindari, Sismindari
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germplasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on growth, leaf proline content and protein pattern of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accession BOT-53 but drought-inducible expressed in BOT-54. Keywords: Drought stress, glagah, proline, protein pattern, Saccharum spontaneum
Inhibition of Klebsiella pneumoniae adhesion in mice enterocytes by antibodies of hemagglutinin pili protein with MW 12.8 kDa of Klebsiella pneumoniae Agustina, Dini; Retoprawiro, Sumarno; AS, Noorhamdani
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Klebsiella pneumoniae as one of the most common cause of VAP is also the second most common cause of community- and hospital-acquired gram negative bloodstream infection. The process of infection caused by direct contact with infectious agents begins with the host cell adhesion process either by pili or by afimbria adhesin (AFA). There were no reported researches on the hemagglutinin pili protein of K.pneumoniae as adhesion factors.This study was conducted in order to determine the hemagglutinin pili protein of K.pneumoniae, polyclonal antibody produced from pili protein immunization, and its ability to inhibit K.pneumoniae adhesion in mice enterocytes. Adhesion inhibition test used HA antibody with the implementation of dose dilutions of 1/100, 1/200, 1/400, 1/800, 1/1600, 1/3200 and 0 (control). While immunocytochemistry test used HA pili protein with the implementation of dose dilutions of 1/10000, 1/20000, 1/40000, 1/80000, 1/160000, 1/320000 and 0 (control). Hemagglutinin pili protein found in K.pneumoniae had MW 12.8 kDa. Pearson correlation analysis of adhesion test showed there were significant correlation between antibody dilution titer with bacterial adhesion (p = 0.032, R = -0.797). Furthermore, Anova analysis of IT showed that there were significant differences between the various dilution titer with antigen-antibody reaction (p=0.000). Antibody of hemagglutinin pili protein with MW 12.8 kDa of K.pneumoniae can inhibit the adhesion of K.pneumoniae to the enterocytes of mice.
Ecotourism and Climates changes: the ecolodge contribution in global warming mitigation Hakim, Luchman; Nakagoshi, Nobukazu
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Global attention to the global warming reduction has invite numerous strategy implemented with the objectives is mitigating greenhouse gasses emission which threats to the future of living in biosphere. Essentially, absorbing CO2 from atmosphere and sequestering in terrestrial ecosystem is one of the significant strategy. While in developing countries it is become essential, support for forest conservation, afforestation and effort to increase terrestrial ability to capture and storage carbon is poor. Ecotourism offer potential key to solved such problems by promoting ecolodge as a sustainable tourism accomodations. This paper aims to explore the potential of ecotourism sector to alleviate global warming and establishing framework for ecolodge planning and development in tropical developing countries. This paper highlight the significant of ecolodge attraction and development management to meet proper carbon capture and sequestration mechanism. The attraction management and developing programs ultimately able to increase plants biomass while accommodation able to practicing energy efficient and optimizing reuse and recycle approach. It will become the potential solution for reducing greenhouse gas emissions and create clean development strategy.
Wild Musa Species Collection of Purwodadi Botanic Garden: Inventory and Its Morpho-taxonomic Review Hapsari, Lia
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Indonesia, being part of the center of origin of bananas (Musaceae), has a large number diversity of bananas both wild seeded species and edible seedless cultivated varieties. Inventory of wild Musa species in Purwodadi Botanic Garden has been conducted through compiling data records from PBGs Registration section, field inspection and observation to living collections in the garden, herbarium specimens and literature studies. The results show that total 17 wild Musa accessions has been recorded planted in Purwodadi Botanic Garden since 1990 until 2012; comprises of 8 Musa acuminata sub species, 2 Musa balbisiana forms, 1 Musa ornata, 1 Musa troglodytarum, 1 Musa borneensis and 4 unidentified species Musa spp.; but only 8 living accessions remained in 2012. Morpho-taxonomic review of those 8 wild Musa accessions remained will be discussed in this paper including their geographical distributions. According to its differentiated morphological characteristics observations, it is known that there are three accessions were resembled cultivars and one unidentified species have been determined its species level, so that their registration identity needs to be revised. It is important next to prioritize ex-situ conservation of wild Musa species not yet collected in Purwodadi Botanic Garden especially from Eastern Indonesia.
Black Kite Populations Are Suffering Declining Trends In Kurukshetra And Likely To Get Further Depleted: An Analysis Of Causes Kaushik, Tirshem Kumar; Gupta, Rohtash Chand
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Black Kite Milvus migrans was seen capturing and captivating everything nearby the offal and garbage sites in Kurukshetra environs until recent past. Today it has been observed that between 2000 and 2010, its depletion is speeding fast, although not noticed in the scientific and social world. The skies in late morning hours are empty. Garbage sites demonstrate the absence of criss-crossing flights of this Kite. The popular sites of the roosting in the shape of Peepal Ficus religiosa, Banyan Ficus benghalensis and Mango Mangifera indica trees have been cut down in the first place. In Kurukshetra, the prime reason for the depletion could be attributed to destruction of foraging grounds (Elimination of garbage dumping sites), cutting down of traditional roosting and nesting sites and construction of colonies in the nearby erstwhile agriculture fields. The contiguous agriculture fields are devoid of any traditional trees like Peepal, Mango and hence no roosting and nesting places. The soaring Kites in the high skies in the forenoon and afternoon sessions were more or less absent. So much so that huge Garbage sites at Panipat Railway compound had no Black Kites. Shockingly, it seems that like Vultures, Black Kites are on their way to elimination in Haryana.
Interaction Of Platelet Activating Factor Acetyl Hydrolase (Paf Ah) Enzyme In Gln281 To Arg281 Mutation Toward Paf And Its Molecular Dynamic Putri, Jayarani Fatimah; ., widodo; Rohman, Muhammad Saifur
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Platelet Activating Factor Acetyl Hydrolase (PAF AH) or LpPLA2 is key enzyme in myocardial infarction catalyzes the sn-2 acetyl group of Platelet Activating Factor (PAF) into lyso PAF and acetate as non-potent inflammatory molecules. PAF AH plays a critical role in arterial plaque development of Coronary Artery Disease (CAD). A crystal structure of PAF AH complexes with other ligand and effects of amino acid alteration to protein plasma consequence have also been reported. Here we report on the result of molecular docking and Molecular dynamic (MD) simulation carried out for PAF AH wild type (WT)/PAF and mutant Q281R/PAF complexes. Docking result shown that amino acid residues on active site of Q281 PAF AH mutant have not recognized on PAF AH. Eelectrostatics and hydrophobic bonds significantly reduced in Q281R than wild type. In the 7500 ps MD simulation Q281R showed less dynamics than WT but enzymatic machinary of mutant Q281R was not interrupted during MD simulation as well as PAF AH wildtype. These findings clearly indicated the importance effect of mutant Q281R in PAF AH recognition to its substrate
Changes in Organic, Inorganic contents, Carbon Nitrogen ratio in decomposingAvicennia marinaand Rhizophora mucronataleaves on tidal mudflats in Hajambro creek, Indus delta, Pakistan Farooqui, Zafar; Siddiqui, Pirzada Jamal; Rasheed, Munawwer
Journal of Tropical Life Science Vol 4, No 1 (2014)
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Abstract

Leaf decomposition of Avicennia marina (Forskål) Vierh in Denkschr and Rhizophora mucronata (Poiret) was studied in situ using litterbags in Hajambro creek, Indus deltA. A single exponential model was presented, which best described the rate of decomposition for both the species. The rate of decomposition was species specific, A. marina leaves decomposed slower than the R. mucronata (p
Haplotype Diversity of COI Gene of Hylarana chalconota Species Found at State University of Malang Wulandari, Dian Ratri; Ibrohim, Ibrohim; Listyorini, Dwi
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.1.%x

Abstract

Hylarana chalconota is a cryptic species of frog endemic to Java Island . This species is small with long legs, and brown skin. The Snout-Vent Length (SVL) ranges between 30-40 mm for male and 45-65 mm for female. Reports the existence of this species in State University of Malang, which was not found in 1995. To reveal the haplotype diversity of COI gene in this species, we analyzed Cytochrome-c oxidase subunit-1 (COI) sequences of both samples. The both samples had 604 bp and 574 bp fragment length, respectively. These fragments showed polymorphism; with mutation position in sites 104, 105, and 124. Based on this result, we suggest that the two samples share a different haplotypes, proposed as UM1 and UM2
Endophytic Bacteria as Biocontrol Agents of Tomato Bacterial Wilt Disease Purnawati, Arika
Journal of Tropical Life Science Vol 4, No 1 (2014)
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Abstract

Ralstonia solanacearum is one important pathogen on tomato because it caused wilt disease that can loss yield until 100%. The object of these experiment are to select of endophytic bacteria that was isolated from healthy tomato stems, root and to investigate of them as biocontrol agents for tomato bacterial wilt disease. There are 10 isolates successfully isolated and two isolates are Ps1 and Ps8 can inhibit of R. solanacearum. The result show the two isolates are Ps1 and Ps8 significantly suppress disease incidence until 8.07-9.19%.
CD4+CD25+ T cells maintain homeostasis by promoting TER-119 cell development and inhibiting T cell activation Rifai, Muhaimin
Journal of Tropical Life Science Vol 4, No 1 (2014)
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CD4+CD25+ regulatory T cells involved in the regulation of self-tolerance and normality of homeostasis. CD122 deficient mice are model animals that have an abnormal immune system characteristically have a high number of activated T cells and TER-119 cell decreased. Here we showed evidence that the transfer of CD4+CD25+ regulatory T cells derived from normal mice to CD122-defficient neonates prevent the development of activated memory T cells and elicit TER-119 differentiation. Bone marrow reconstitution derived from CD122-/- mice to normal mice resulting tolerance to individual that genetically different. Importantly, CD4+CD25+ regulatory T cells derived from normal mice can replace CD4+CD25+ cells derived from CD122-/- mice. The results of this experiment suggest that regulatory T cells from normal mice exert a critical role in maintaining peripheral tolerance and controlling hematopoietic disorder.

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